3. Enzymology

Enzyme Mechanisms — Quiz

Test your understanding of enzyme mechanisms with 5 practice questions.

Practice Questions

Question 1

Which amino acid residue is most commonly utilized in general acid-base catalysis due to its side chain pKa being near physiological pH?

Question 2

On an enzyme reaction coordinate diagram, the highest point along the reaction pathway corresponds to which species?

Question 3

For an enzyme with k_cat=150\,$\mathrm{s^{-1}}$ and K_M=30\,$\mu$$\mathrm{M}$, what is the specificity constant k_cat/K_M in $\mathrm{M^{-1}\,s^{-1}}$?

Question 4

If an enzyme stabilizes the transition state by 50\,$\mathrm{kJ/mol}$ relative to the uncatalyzed reaction, what is the approximate fold increase in reaction rate at 300\,$\mathrm{K}$, given rate enhancement $\approx$ e^{$\Delta$$\Delta$ G^\ddagger/(RT)}?

Question 5

In carbonic anhydrase, the Zn^{2+} ion most directly facilitates catalysis by: