5. Molecular Genetics
Recombinant Methods — Quiz
Test your understanding of recombinant methods with 5 practice questions.
Practice Questions
Question 1
Which advantage does overlap extension PCR offer over traditional restriction-ligation cloning?
Question 2
In ligation-independent cloning (LIC), which activity of T4 DNA polymerase is exploited to generate single-stranded overhangs?
Question 3
Using the ΔΔCT method in quantitative PCR, if a sample has ΔCT=3 and control has ΔCT=5, what is the fold change in expression?
Question 4
What is a key advantage of delivering CRISPR–Cas9 as a ribonucleoprotein (RNP) complex rather than via plasmid?
Question 5
Why is periplasmic targeting of recombinant proteins in E. coli often beneficial for proteins requiring disulfide bonds?