6. Techniques and Applications
Chromatography — Quiz
Test your understanding of chromatography with 5 practice questions.
Practice Questions
Question 1
Which key property of an analyte determines whether gas chromatography is an appropriate analytical method?
Question 2
Which modification to a size-exclusion chromatography column would most increase resolution between molecules of similar size?
Question 3
Given an HPLC chromatogram with retention times $t_{R1}=3.0\,\mathrm{min}$, $t_{R2}=4.5\,\mathrm{min}$, and dead time $t_{0}=0.5\,\mathrm{min}$, what is the selectivity factor $\alpha= k_2/k_1$?
Question 4
To improve separation between two closely eluting peaks in HPLC, which adjustment is most effective?
Question 5
Which affinity chromatography technique uses immobilized antibodies to selectively purify a target protein?