Which of the following computational challenges is most significant when attempting to assemble a $de novo$ genome from short-read sequencing data, particularly in organisms with large and complex genomes?
Question 2
In the context of high-throughput sequencing, what is the primary advantage of using long-read sequencing technologies (e.g., PacBio, Oxford Nanopore) over traditional short-read platforms for $genome assembly$?
Question 3
A researcher is performing $comparative genomics$ between a pathogenic bacterium and a non-pathogenic closely related strain. They identify several genes present in the pathogenic strain but absent in the non-pathogenic one. What is the most likely functional category for these unique genes?
Question 4
In $precision medicine$, a patient presents with a rare form of cancer. $Whole-exome sequencing$ reveals a novel somatic mutation in a gene known to be involved in cell proliferation. Before prescribing a targeted therapy, which of the following steps is most crucial to ensure the mutation is $actionable$?
Question 5
Which of the following best describes the primary role of $bioinformatics tools$ in $genome assembly$ from high-throughput sequencing data?
