6. Applications & Emerging Areas
Capstone Project — Quiz
Test your understanding of capstone project with 5 practice questions.
Practice Questions
Question 1
A biotechnology capstone project involves developing a novel therapeutic protein. Which of the following post-translational modifications is most critical to consider for ensuring the protein's stability, activity, and immunogenicity in a human physiological context?
Question 2
In a biotechnology capstone project focused on developing a gene editing therapy for a monogenic disorder, the team is evaluating different delivery methods for CRISPR-Cas9 components. Which of the following non-viral delivery systems offers the best balance of safety, packaging capacity for large constructs, and potential for repeated administration in vivo?
Question 3
A capstone team is designing a high-throughput screening (HTS) assay to identify small molecule inhibitors of a specific enzyme. They are considering a fluorescence resonance energy transfer (FRET) based assay. Which of the following experimental parameters is most crucial to optimize to ensure a robust and reliable HTS assay with minimal false positives/negatives?
Question 4
A biotechnology capstone project aims to develop a cell-free protein synthesis (CFPS) system for rapid prototyping of therapeutic proteins. Which of the following modifications to the CFPS extract would most significantly enhance protein yield and stability by reducing protease activity and extending reaction duration?
Question 5
A capstone team is developing a CRISPR-based diagnostic tool for rapid detection of viral RNA. They are utilizing a Cas13 enzyme. Which of the following characteristics of Cas13, compared to Cas9, makes it uniquely advantageous for this diagnostic application?