2. Genetic Engineering

Vectors And Hosts — Quiz

Test your understanding of vectors and hosts with 5 practice questions.

Practice Questions

Question 1

When designing a plasmid vector for stable maintenance and replication in a bacterial host, which of the following features is most critical for ensuring its propagation across cell divisions?

Question 2

A researcher aims to express a complex human protein that requires specific post-translational modifications, such as glycosylation, for its biological activity. Which host system would be the most appropriate choice to ensure these modifications occur correctly?

Question 3

In the context of gene therapy, a major concern with using certain viral vectors is the potential for insertional mutagenesis. Which type of viral vector is most commonly associated with this risk due to its mechanism of action?

Question 4

When designing an expression vector for tightly regulated, high-level protein production in $\textit{E. coli}$, which type of promoter would be the most effective choice?

Question 5

A researcher is working with a gene that encodes a highly toxic protein, and its continuous expression is lethal to the bacterial host. Which characteristic is most important when selecting a host strain for cloning and expressing this particular gene?