4. Plant Pathology

Diagnostics — Quiz

Test your understanding of diagnostics with 5 practice questions.

Practice Questions

Question 1

Which temperature range does Recombinase Polymerase Amplification (RPA) require for isothermal pathogen detection in the field?

Question 2

When performing nested PCR to detect low‐titer plant pathogens, what is the primary risk if amplicons from the first round contaminate the second‐round reaction?

Question 3

To estimate a pathogen incidence of 10% with a margin of error of ±5% at 95% confidence, what sample size $n$ is required using the approximation $n=\frac{p(1-p)z^2}{E^2}$ with $z=1.96$?

Question 4

A greenhouse tomato crop exhibits irregular chlorotic mosaic patterns on upper leaves and concentric ringspots on fruit, without bacterial ooze or fungal sporulation. Which causal agent group is most likely?

Question 5

Which advantage does droplet digital PCR (ddPCR) have over quantitative PCR (qPCR) for pathogen quantification?