6. Immunotherapy and Research Methods

Laboratory Techniques — Quiz

Test your understanding of laboratory techniques with 5 practice questions.

Practice Questions

Question 1

In a flow cytometry experiment using counting beads at a concentration of 50\,000 beads/mL, a sample yields 1000 bead events and 4000 target cell events. What is the concentration of target cells in cells/mL?

Question 2

When performing multicolor flow cytometry, what is the primary consequence of setting a PMT voltage too high for a given detector?

Question 3

Which ELISA format is most appropriate for detecting a low-abundance cytokine in serum while minimizing non-specific binding?

Question 4

In a sandwich ELISA standard curve described by the linear equation $A=0.05+0.1C$ where $A$ is absorbance and $C$ is cytokine concentration in ng/mL, what is the concentration of a sample with $A=0.55$?

Question 5

For optimal resolution of proteins at 25 kDa and 150 kDa in SDS-PAGE prior to Western blotting, which gel composition is most suitable?