6. Immunotherapy and Research Methods

Monoclonal Antibodies — Quiz

Test your understanding of monoclonal antibodies with 5 practice questions.

Practice Questions

Question 1

In CHO cell–based fed‐batch cultures for monoclonal antibody production, what is the primary benefit of the fed‐batch approach?

Question 2

Protein A affinity chromatography is widely used in mAb purification because Protein A binds specifically to which region of the antibody?

Question 3

In the design of antibody–drug conjugates (ADCs), linkers that are cleavable by lysosomal proteases are engineered to remain stable in plasma and be cleaved under what condition?

Question 4

Surface plasmon resonance (SPR) analysis yields equilibrium dissociation constants (K_D). A mAb with $K_D=1\times10^{-9}\,\mathrm{M}$ compared to one with $K_D=1\times10^{-6}\,\mathrm{M}$ is described as:

Question 5

A monoclonal antibody that binds a cell surface receptor and prevents its natural ligand from binding is acting as a(n):