3. DNA Metabolism

Dna Replication — Quiz

Test your understanding of dna replication with 5 practice questions.

Practice Questions

Question 1

Which protein complex acts as the eukaryotic replicative helicase that unwinds DNA at replication forks?

Question 2

Which subunit of the E. coli DNA polymerase III holoenzyme physically connects the polymerase core enzymes to the clamp loader?

Question 3

Which enzyme relieves positive supercoiling ahead of the replication fork by transiently introducing double-strand breaks in the DNA?

Question 4

During maturation of Okazaki fragments in eukaryotes, which two activities sequentially remove RNA primers and process flap structures for subsequent ligation?

Question 5

If replication forks progress at $800\\ \mathrm{nt/s}$ in a bacterium with a single circular chromosome of $3.2\times10^6\\ \mathrm{bp}$, how many minutes are required for complete replication? Assume two replication forks. Show your calculation.