5. Gene Regulation

Synthetic Regulation — Quiz

Test your understanding of synthetic regulation with 5 practice questions.

Practice Questions

Question 1

Which design principle in synthetic gene circuits primarily reduces stochastic noise in gene expression?

Question 2

When engineering two activator-binding sites on a bacterial promoter to enable cooperative binding, what nucleotide spacing should maximize helical phasing?

Question 3

A promoter is constructed with two distinct activator-binding sites upstream of a minimal core promoter such that binding of either activator X or activator Y initiates transcription. Which Boolean logic function does this implement?

Question 4

In CRISPR activation using dCas9–VP64, targeting the sgRNA to which region relative to the transcription start site (TSS) generally yields maximal activation?

Question 5

Given a CRISPR interference system with dCas9–sgRNA dissociation constant $K_d=20\,\mathrm{nM}$, what fraction of target sites is occupied when the dCas9–sgRNA concentration is $5\,\mathrm{nM}$? Show your calculation.