Question 1
In de novo genome assembly using de Bruijn graphs, what do nodes and edges represent?
Question 2
A sequencing read of length 100 bases is processed with k-mer size $k=25$. How many k-mers are generated from this read? Show your calculation.
Question 3
Which of the following best distinguishes de novo genome assembly from reference-guided assembly?
Question 4
In RNA-Seq library preparation, which step specifically enriches for mRNA transcripts compared to genomic DNA library prep?
Question 5
In a profile hidden Markov model (HMM) for protein domain detection, which set of states is foundational?
