6. Techniques

Cloning Methods — Quiz

Test your understanding of cloning methods with 5 practice questions.

Practice Questions

Question 1

Why can the dephosphorylation step be omitted when digesting a plasmid vector with two different restriction enzymes and performing preparative gel purification?

Question 2

What length of homologous overlap is typically required at each end of DNA fragments for efficient Gibson Assembly?

Question 3

Which limitation is commonly associated with Gibson Assembly?

Question 4

In Gibson Assembly, which enzyme is responsible for filling in single-stranded gaps by synthesizing DNA complementary to the overhangs?

Question 5

In Gateway Cloning, an Entry Clone contains the gene of interest flanked by which recombination sites?