6. Techniques

Sequencing — Quiz

Test your understanding of sequencing with 5 practice questions.

Practice Questions

Question 1

What is the main function of the end repair step in Illumina library preparation?

Question 2

A sequencing run yields 150 million paired-end reads of 150 bp each for a 3 Gbp genome. What is the approximate sequencing coverage? Show your calculation.

Question 3

What does a mapping quality (MAPQ) score of 0 indicate for an aligned read?

Question 4

Why is marking PCR duplicates important in sequencing data analysis?

Question 5

Which file format stores aligned reads in a compressed binary form and requires an index for efficient region-specific access?